Purification of Baculovirally Expressed FLAGTagged TR and RXR from Sf9 Cells

1. pVL1392-FLAG-TR and pVL1392-FLAG-RXR plasmid DNA (see Note 3).

2. Sf9 Insect cells (Invitrogen, Carlsbad, CA).

3. Grace's insect medium (Life Technologies, cat. no. 11605-102).

4. 10% Pluronic F-68 solution (Sigma, cat. no. P5556).

5. Gentamycin (10 mg/mL) (Life Technologies, cat. no. 15710-064).

6. BaculoGold-modified baculovirus DNA (Pharmingen, San Diego, CA, cat. no. 21100D).

7. Insectin-Plus cationic liposomes (Invitrogen, cat. no. K3695-01).

8. Coomassie blue stain: dissolve 0.25 g of Coomassie Brilliant Blue R250 (Sigma) in 90 mL of methanol:ddH2O (1:1 v/v) and 10 mL glacial acetic acid.

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