GSTBinding Reactions

1. 35S-labeled in vitro translated RTH-TRs (see Subheading 2.2.).

2. Phosphate-buffered saline with Triton (PBST) Buffer: 150 mM NaCl, 16 mM Na2HPO4, 4 mM NaH2PO4, 2 mM EDTA, 1% Triton X-100, 1 mM DTT.

3. HEMG buffer: 40 mM HEPES, pH 7.5, 100 mM KCl, 0.2 mM EDTA, 5 mM MgCl2, 0.1% NP-40, 10% glycerol, 1.5 mM DTT.

4. Glutathione-coupled agarose (Sigma, St. Louis, MO).

5. 10 mM Glutathione in 50 mM Tris-HCl, pH 8.0.

6. 100 mg/mL bovine serum albumin (BSA).

7. Complete protease inhibitor cocktail (Roche, IN).

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