Generation of Hypothyroid Hyperthyroid and Euthyroid Mice

Hypothyroid mice are generated by feeding them with a low iodine (loI) diet supplemented with 0.15 propylthiouracil (PTU) purchased from Harlan Teklad Co. (Madison, WI) for 4 wk. 2. Serum thyroid-stimulating hormone (TSH) measurements (kindly measured by Dr. Samuel Refetoff, University of Chicago, Chicago, IL) show that mice littermate control and transgenic mice treated with PTU were profoundly hypothyroid. 3. Hyperthyroid mice are generated by injecting hypothyroid mice intraperitoneally with...

NCoRi Animal Model to Study Biological Function of NCoR in Liver

Hollenberg et al. (37) have reported that a variant form of NCoR, NCoRi, lacks the repression domains in the amino terminus, but retains the nuclear receptor interaction domains. NCoRi is derived from a 3.1 kb cDNA that was originally isolated from a human placental library. NCoRi protein contains the TR interaction domains and surrounding amino acids (AA 1539-2453), but lacks the repressor domains (AA 1-1120) present in full-length murine NCoR. It also has been shown that NCoRi has dominant...

Generation of Hypothyroid Rats and Mice

The induction of severe hypothyroidism is essential to observe changes in gene expression by thyroid hormone. Moderate hypothyroidism leads to physiological changes aimed at maintaining T3 concentrations in neural tissue within normal levels. The most important mechanism concerns deiodinase type 2 (D2). D2 is a selenoenzyme that catalyzes the removal of the iodine atom in the 5' position of T4 to generate the active hormone, T3. D2 activity is inhibited by T4 through a mechanism involving...