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Electron Microscopy of Macromolecular Assemblies

Figure 2.1 Schematic diagram of a transmission electron microscope. Adapted from a drawing provided by Roger C. Wagner, University of Delaware (Web site www.udel.edu Biology Wags b617 b617.htm). Figure 2.1 Schematic diagram of a transmission electron microscope. Adapted from a drawing provided by Roger C. Wagner, University of Delaware (Web site www.udel.edu Biology Wags b617 b617.htm).

Principle of the Transmission Electron Microscope

For the general principle and theory of scattering and image formation, and the many applications of the transmission electron microscope, the reader is referred to the authoritative works by Reimer (1997) and Spence (1988). In the following, we will make reference to a schematic diagram of a typical transmission electron microscope (figure 2.1) to explain the essential elements.

Results And Discussion

Schematic diagram representing the recovery of rIBV from DNA isolated from a rVV containing a full-length IBV cDNA under the control of a T7 promoter. A plasmid expressing the IBV nucleoprotein is required for successful rescue of IBV. The infectious IBV RNA is generated using T7 RNA polymerase expressed from fowlpox virus.

Tactile Learning In A Topographic Framework

FIGURE 7.2 Schematic diagram illustrating two alternative models for the cortical distribution of sensory learning. On the left, an orderly array of sensory receptors projects to an orderly array of cortical processing zones, which correspond to storage sites for learned information. On the right, the cortical processing and storage zones are widely distributed, highly overlapping, and do not conserve topographic order. Experiments that probe the patterns of learning transfer to nearby sensory receptors after training with one restricted site can discriminate between these models. FIGURE 7.2 Schematic diagram illustrating two alternative models for the cortical distribution of sensory learning. On the left, an orderly array of sensory receptors projects to an orderly array of cortical processing zones, which correspond to storage sites for learned information. On the right, the cortical processing and storage zones are widely distributed, highly overlapping, and do not conserve...

Modelling geometric constraint

A theoretical hyperdimensional space of possible form Each dimension of the space represents a morphological trait that may be measured on a given biological form, f. All possible coordinate combinations within the hyperdimensional space represent the set of all possible biological forms. Although only eight dimensions are shown in this schematic diagram, the dimensionality of an actual hyperspace of form will be much larger.

Of Behavior of CTermini in Microtubules

Left A schematic diagram of portions of two microtubules connected by a MAP2. Each portion shows the outer face of a tubulin dimer covered by two C-termini. Each C-terminus can be in one of two metastable states, perpendicular or parallel to the dimer surface. Right A portion of the microtubule composed of a few tubulin dimers with their C-termini used as a model for the static simulation (right). Also shown are a few of the positively charged regions (circles) on the surface of a tubulin dimer. The test C-terminus is slanted (bold) Fig. 8.5. Left A schematic diagram of portions of two microtubules connected by a MAP2. Each portion shows the outer face of a tubulin dimer covered by two C-termini. Each C-terminus can be in one of two metastable states, perpendicular or parallel to the dimer surface. Right A portion of the microtubule composed of a few tubulin dimers with their C-termini used as a model for the static simulation (right). Also shown are a few of the positively...

Hydrocolloid Functionality In Foods 921 Thickening Properties

Figure 9.1 Schematic diagram of polymer coils in solution illustrating (a) dilute solution conditions where the polymer coils are free to move independently (cc*). Figure 9.1 Schematic diagram of polymer coils in solution illustrating (a) dilute solution conditions where the polymer coils are free to move independently (cc*).

Cox enzymes and prostanoid synthesis

Figure 1 shows schematic diagram of prostanoid synthesis by COX-1 and COX-2. Upon physiological signals, stress, or injury, cells produce prostanoids via enzymatic metabolism of arachidonic acid, a polyunsaturated fatty acid bound as an ester to cell membrane phospholipids. Arachidonic acid is first liberated from the membrane phospholipids by phospholipase A2 and then catalyzed by COX enzymes to prostaglandin (PG) G2. Being

Neural Representations

Schematic diagram showing the main brainstem pathways of the central auditory system. Crossed monaural pathways are shown in black binaural pathways are indicated in gray. CN, cochlear nucleus DNLL, dorsal nucleus of the lateral lemniscus IC, inferior colliculus SOC, superior olivary complex INLL, intermediate nucleus of the lateral lemniscus VNLLc, ventral nucleus of the lateral lemniscus, columnar division VNLLm, ventral nucleus of the lateral lemniscus, multipolar cell division (from Covey, 2001). Schematic diagram showing the main brainstem pathways of the central auditory system. Crossed monaural pathways are shown in black binaural pathways are indicated in gray. CN, cochlear nucleus DNLL, dorsal nucleus of the lateral lemniscus IC, inferior colliculus SOC, superior olivary complex INLL, intermediate nucleus of the lateral lemniscus VNLLc, ventral nucleus of the lateral lemniscus, columnar division VNLLm, ventral nucleus of the lateral lemniscus, multipolar cell division (from...

Combination of a bioreactor with a flow microcalorimeter

Bioreactor Agitator Dimensions

Fig. 1A schematic diagram for the measurement of the heat flow rate of bioreactor-cul-tured animal cells using a flow microcalorimeter. 1 - bioreactor 2 - cultured cells 3 - jacket waier for temperature control in the bioreactor 4 - agitator 5 - the outlet tubing of the jacket water is used for warming the cell suspension in the tubing leading to the microcalorimeter 6 - a non-conductive sponge-plastic pipe is used to reduce heat dissipation along the outlet jacket water attached to the PEEK tubing transmitting the cell suspension to the calorimeter 7 - PEEK T-piece 8 - PEEK two-way valve 9 - PEEK tubing (1 mm I.D.) 10 - glass bottle holding sterilized medium for washing the flow vessel of the microcalorimeter through short-time interruption of the heat flow measurement 11 - sterile medium for cleaning the flow vessel free of possible accumulated animal cells, that would result in an overestimation of the heat flow rate for the cells in the bioreactor 12 - a representation of the...

Pulmonary Vasculature

Schematic diagram of a parabronchus showing its various component parts and the geometric relationships between the air flow and the blood flow. (Maina 2002 c) Fig. 67. Schematic diagram of a parabronchus showing its various component parts and the geometric relationships between the air flow and the blood flow. (Maina 2002 c)

The principles of ohmic heating

Fig. 19.1 A schematic diagram of an ohmic heating device. system that serves as an electrical resistance. Ohmic heating is alternatively called resistance heating or direct resistance heating. The principles of ohmic heating are very simple, and a schematic diagram of an ohmic heating device is shown in Fig. 19.1. During ohmic heating, AC voltage is applied to the electrodes at both ends of the product body. The rate of heating is directly proportional to the square of the electric field strength, the electrical conductivity, and the type of food being heated. The electric field strength can be controlled by adjusting the electrode gap or the applied voltage, while the electrical conductivities of foods vary greatly, but can be adjusted by the addition of electrolytes.

General Observations

Avian Lung

A Cross section of a blood capillary in the lung showing the components through which oxygen diffuses, i.e. the BGB, the plasma layer, and the erythrocyte cytoplasm. B Schematic diagram and an electron micrograph of the lung of the black-headed gull, Larus ridibundus, showing the air-hemoglobin pathway, i.e. the distance that a diffusing oxygen molecule has to traverse. Scale bar B 0.2 m. (Maina 1989b) Fig. 81. A Cross section of a blood capillary in the lung showing the components through which oxygen diffuses, i.e. the BGB, the plasma layer, and the erythrocyte cytoplasm. B Schematic diagram and an electron micrograph of the lung of the black-headed gull, Larus ridibundus, showing the air-hemoglobin pathway, i.e. the distance that a diffusing oxygen molecule has to traverse. Scale bar B 0.2 m. (Maina 1989b)

Structure Determination

Pentameric Capsomeres

Schematic diagram of the papilloma (or polyoma) capsid. 72 pentamers (termed capsomeres) of the major capsid protein L1 are arranged in a T 7 icosahedral lattice. In contrast to the rules of quasi-equivalence described by Caspar and Klug, the capsomeres are only pentamers and not pentamers and hexamers. Thus, pentamers must arrange themselves in two distinct positions hexavalent (six neighboring pentamers) and pentavalent (five neighboring pentamers). The intrinsic bond switching capabilities of the L1 protein make this arrangement possible through changing conformations in the invading C-terminal arms of the protein as they bond with the neighboring pentamer (reproduced from Salunke et al., 1986). Figure 5.1. Schematic diagram of the papilloma (or polyoma) capsid. 72 pentamers (termed capsomeres) of the major capsid protein L1 are arranged in a T 7 icosahedral lattice. In contrast to the rules of quasi-equivalence described by Caspar and Klug, the capsomeres are only...

Classification of Vascular Rings and Related Malformations

Schematic diagram indicating the various components of the embryonic aortic arch complex in the human embryo. Those components which do not precisely persist in the adult Fig. 2.1. Schematic diagram indicating the various components of the embryonic aortic arch complex in the human embryo. Those components which do not precisely persist in the adult

Figure 835

Schematic diagrams of the three 60Co source types, type 1, type 2, and type 3, used with the Ralston remote afterloader (Shimadzu Corporation, Japan) showing geometries and materials. The coordinate system used for published data36 coincides in each case with the corresponding center of symmetry of the two active pellets and it is also shown for the type 1 source design. All dimensions shown are in mm.

Figure 850

Schematic diagram of the CIS CSM11 type (CIS Bio International, France) low dose rate 137Cs cylindrically shaped source.39 The origin of the coordinate system is in the center of the cylindrical active core, where the + z direction is aligned towards the round tip-end of the source (right direction in the figure). The origin was assumed by Ballester et al.39 at the center of the source and not the center of the active core length (displacement of 0.05 cm).

Conclusions

A schematic diagram showing positive and negative regulation of cartilage growth by the perichon-drium (PC), the periosteum (PO), and the articular perichondrium (APC). As shown on the left hand side of the figure, the PC and PO themselves each independently stimulate growth, as does the APC (shown at the top). However, when the PC and PO act together they effect precise negative regulation. As shown on the right, both TGF- 31 and RA act on the perichondrium, to induce this tissue to produce a factor (or factors) that also effect precise negative regulation. It seems likely that these different forms of negative regulation of growth predominate over the positive stimulation however, this remains to be tested experimentally. Fig. 11. A schematic diagram showing positive and negative regulation of cartilage growth by the perichon-drium (PC), the periosteum (PO), and the articular perichondrium (APC). As shown on the left hand side of the figure, the PC and PO themselves each...

Gelling Properties

Figure 9.3 Schematic diagram of the polymer network structure of a hydrocolloid gel with noncovalently bonded ordered junction zones linking the polymer chains into a three-dimensional network. Figure 9.3 Schematic diagram of the polymer network structure of a hydrocolloid gel with noncovalently bonded ordered junction zones linking the polymer chains into a three-dimensional network.

Surface Rendering

The schematic diagram in figure 6.8 explains how in general the distance of the surface from the viewer is derived from the 3D density distribution stored in the volume. First, a threshold density d0 must be defined, to create the distinction between ''inside'' (d d0) and ''outside'' (d

Temporal Resolution

Schematic diagram of a two-interval, forced-choice psychophysical paradigm used to estimate gap detection thresholds (top row) and sinusoidal amplitude modulation (SAM) detection thresholds (bottom row). Stimulus waveforms are shown for each of two observation intervals. A broadband noise standard is shown in interval 1 and a noise with a temporal gap (64 ms) or amplitude modulation (6 dB) is shown in interval 2. Correct and incorrect responses are listed. Figure 1. Schematic diagram of a two-interval, forced-choice psychophysical paradigm used to estimate gap detection thresholds (top row) and sinusoidal amplitude modulation (SAM) detection thresholds (bottom row). Stimulus waveforms are shown for each of two observation intervals. A broadband noise standard is shown in interval 1 and a noise with a temporal gap (64 ms) or amplitude modulation (6 dB) is shown in interval 2. Correct and incorrect responses are listed.

Ostium In Avian

Schematic diagram of the lung-air sac system of birds showing the primary bronchus (PB) running through the lung. In transit, it gives rise to medioventral secondary bronchi (MVSB), and mediodorsal secondary bronchi (MDSB). The secondary bronchi are connected by different groups of parabronchi, namely the paleopulmonic parabronchi (PPPB) and neopulmonic parabronchi (NPPB). Arrows Ostia AAS abdominal air sac CaTAS caudal thoracic air sac CrTAS craniothoracic air sac ClAS clavicular air sac CeAs cervical air sac. (Redrawn from Duncker 1974, with permission from the publisher) Fig. 66. Schematic diagram of the lung-air sac system of birds showing the primary bronchus (PB) running through the lung. In transit, it gives rise to medioventral secondary bronchi (MVSB), and mediodorsal secondary bronchi (MDSB). The secondary bronchi are connected by different groups of parabronchi, namely the paleopulmonic parabronchi (PPPB) and neopulmonic parabronchi (NPPB). Arrows Ostia AAS...

Control of Air Flow

Secondary Air Flow 2002 Seville

Schematic diagram showing acceleration of air flow past the constriction of the extrapulmonary primary bronchus at the segmentum accelerans, resulting in the shunting of air past the medioventral secondary bronchi, i.e. inspiratory aerodynamic valving. (Maina 2002 c) Fig. 76. Schematic diagram showing acceleration of air flow past the constriction of the extrapulmonary primary bronchus at the segmentum accelerans, resulting in the shunting of air past the medioventral secondary bronchi, i.e. inspiratory aerodynamic valving. (Maina 2002 c) Fig. 79. Schematic diagram of the lung of the ostrich, Struthio camelus, showing measurements and geometries of some of the air-conducting airways. (Maina and Nathaniel 2001) Fig. 79. Schematic diagram of the lung of the ostrich, Struthio camelus, showing measurements and geometries of some of the air-conducting airways. (Maina and Nathaniel 2001)

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