Methods

The anatomical methods used in this experiment have been previously described (Boire et al., 1995) and will be only briefly presented here. Four kittens at 12 days old, under deep general anesthesia, underwent the splitting of the optic chiasm (OC) using a transbuccal approach (Boire et al., 1995). They were then raised in the animal colony for about 759 days. They received large unilateral injections of horseradish peroxydase (40% HRP, 10—12 0.5-ml injections) in the visual cortex so as to retrogradely label the callosally projecting neurons in the opposite hemisphere (Innocenti and Frost, 1980). Following a two-day survival period, the animals were deeply anesthetized and transcardially perfused. The brains were then dissected, stored overnight in 30% su

Figure 1.9. Distribution of HRP-labeled callosal neurons callosal cells in the supragranular layers compared to the in-in coronal sections through areas 17 and 18 of one normal fragranular ones. (N5) and one split chiasm cat (OCS 2). Note the abundance of

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