Figure 2. (a) Adenoviral transfection of luciferase reportergene DNA revealed a modest effect after antegrade infusion into the region of interest (LAD-perfused area), even though the venous outflow was blocked by a balloon in the anterior interventricular vein). (b) Retroinfusion itself, however, increased reportergene expression in the corresponding area significantly (modified from Boekstegers et al., Gene Therapy 2000). (c) In comparison to the percutaneous intramyocardial delivery (PIMD) using a needle catheter, or surgical intramyocardial delivery (SIMD) after lateral thoracotomy, percutaneous retrograde delivery of adenoviral galactosidase revealed a more homogenous and in part more efficient transfection mode
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