Procedure for Measuring Transepidermal Water Loss

To avoid the influence of menstruation and associated humidity and fluid effects on SSWL, the investigators measured TEWL 9 to 11 days post menses. The measurements were taken in a quiet room and under constant environmental conditions (29°C to 32oC at skin surface, ambient at 21°C to 23°C, and 41% to 58% relative humidity) using an evaporimeter over a 30-minute period (data collected after 0, 2, 5, 10, 20, and 30 minutes). Following the vulvar measurements, TEWL measurements were made at an adjoining thigh area. Only data collected from participants with a negative rate of TEWL change (n = 54) were used in the statistical analysis.

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