This study confirmed that the SSWL dynamics for vulvar skin in a large participant sampling and its properties are considerably different from an adjacent semi-occluded inner thigh region. To avoid the influence of the menstrual cycle and associated excess humidity and menstrual fluid, we collected data

9 to 11 days following menstruation. Interestingly, the menstrual cycle has been shown to influence skin reactivity to contact irritants at distal locations (i.e., upper arm) (19). As TEWL is believed to be one parameter associated with skin reactivity, we speculate that the menstrual cycle may similarly affect SSWL dynamics at the vulva.

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