This technique specifically stains the pericentromeric regions of all chromosomes, the heterochro-matin regions of chromosomes 1, 9, and 16 and the distal Yq, and the satellites of the acrocentric chromosomes. An alkaline treatment of the chromosomes causes loss of the Giemsa binding sites. Optimal results are achieved at pH 11.6. At this high alkaline pH, only the azure component of Giemsa binds with the majority of the chromosomes, staining them light blue. The eosin component of Giemsa binds specifically to the heteromorphic regions cited above, staining them magenta. G-11 banding is used to delineate these heterochromatin polymorphisms.
G-11 banding also has research applications. It is used to differentiate between human and rodent chromosomes in hybrid cells. The human chromosomes stain pale blue, whereas the rodent chromosomes stain magenta.
Was this article helpful?
Far too many people struggle to fall pregnant and conceive a child naturally. This book looks at the reasons for infertility and how using a natural, holistic approach can greatly improve your chances of conceiving a child of your own without surgery and without drugs!